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Overview of the MIBI staining protocol with Ionpath reagents and slides.

Prepare and Bake the Slides
Ionpath Reagent:

• MIBI Slides (Cat #: 567001)

Deparaffinization and Rehydration
Ionpath Reagent:

• MIBI diH2O (Cat#: 567002)

Heat-Induced Antigen Retrieval

Wash
Ionpath Reagent:

• MIBI 20X TBS-T (Cat#: 567005)

Blocking

Antibody Staining
Ionpath Reagents:

• MIBI Conjugated Antibodies  (Cat#: variable)

OR

• MIBI Conjugation Kits (Cat#: variable)

Fixation and Dehydration
Ionpath Reagents:

• MIBI 20X TBS-T (Cat#: 567005)
• MIBI 10X PBS (Cat#: 567004)
• MIBI 10X Tris pH8.5 (Cat#: 567003)
• MIBI diH2O (Cat#: 567002)

Dry and Store the Slides