Background: Na/ K-ATPase is a widely expressed membrane protein that functions as an ion pore, exporting sodium and importing potassium. The alpha, beta and FXYD subunits combine to form Na/K-ATPase. The regulation of intracellular concentrations of various ions control signaling pathways and other enzymatic processes, such as neurotransmitter re-uptake in astrocytes. Na/K-ATPase activity is regulated through multiple phosphorylation sites. Mutations in the alpha subunit of Na/K-ATPase, in particular, have been shown to adversely impact ionic homeostasis, contributing to neurological disease.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views.

Recommended Usage: Human FFPE: 1:100 dilution. For optimal results, the antibody should be titrated for each desired application.

References

Clausen, M.V., Hilbers, F. and Poulsen H. (2017) The Structure and Function of the Na,K-ATPase Isoforms in Health and Disease. Front. Physiol. 8:371. doi: 10.3389/fphys.2017.00371

* Conjugate tested on human and mouse FFPE tissue.