Background: Keratin is a fibrous structural protein that assembles into bundles to form intermediate filaments of the cytoskeleton within epithelial cells. The present antibody is a mix of clones AE1 and AE3 that recognize most acidic and basic cytokeratin family members (CK1, 2, 3, 4, 5, 6, 7, 8, 10, 14, 15, 16 and 19). Specific tumor types express these keratins making this a useful marker in the identification of tumor cells and of the origin of a neoplasm.

Validation: Each lot of conjugated keratin antibody is quality control tested by MIBIscope™ analysis of stained tissue microarrays using the appropriate positive and negative tissue field of views and are pathologist verified.

Recommended Usage: 1 uL of keratin antibody per 100 uL staining volume using the MIBI™ Staining Protocol.
For optimal results, antibody should be titrated for each desired application.  Suggested starting range is 1:100.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References

• Jacob, J.T., Coulombe, P.A., Kwan, R., Omary, M.B. Types I and II Keratin Intermediate Filaments. Cold Spring Harbor Perspect Biol. 2018;10(4).

* Conjugate tested on human tissue.