Background: Found in all eukaryotic cells, histones are basic nuclear proteins important for chromatin structure and transcription regulation.  DNA is wrapped around histones into repeating units called nucleosomes.  The accessibility of specific regions of DNA to transcription factors and RNA polymerase II is tightly regulated via post-translational modifications to histones.  Histone  H3  becomes phosphorylated at multiple sites (serine 10 and 28, threonine 11) during mitosis and meiosis.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views. 

Recommended Usage:Human FFPE: 0.2 ug/mL dilution. For optimal results, the antibody should be titrated for each desired application.

References

• Li, Bing et al. The role of chromatin during transcription. Cell. 2007; 128(4):707 – 719.

* Conjugate tested on human tissue.