Background: CD8 is a co-receptor for the T cell receptor (TCR) and is expressed on cytotoxic T cells. It is also expressed on cortical thymocytes, dendritic cells and NK cells. CD8 binds to MHC Class I to aid in antigen recognition and TCR-mediated activation. CD8 forms dimers of CD8ɑ and CD8β and clone D4W2Z recognizes the alpha form of CD8.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis of stained tissue microarray using the appropriate positive and negative tissue field of views. 

Recommended Usage: Mouse FFPE: 5 ug/mL dilution.
For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References
1. Guy, C. S. and Vignali, D. A. Organization of proximal signal initiation at the TCR:CD3 complex. Immunological Reviews. 2009; 232: 7-21.

2. Gao, G. F., Rao, Z., and Bell, J. I. Molecular coordination of αß T-cell receptors and coreceptors CD8 and CD4 in their recognition of peptide-MHC ligands, Trends in Immunology, 2002; 23(8):408-13.

* Conjugate tested on mouse fresh frozen tissue.