Background: CD3 is found on all mature T cells, serving important roles for T cell development. CD3 is part of the T cell receptor that recognizes antigen, resulting in the activation of signaling pathways. During T cell development the intensity of the response to antigen is tightly controlled to select for responsive T cells that are not autoreactive. In disease, the lack of CD3 expression is associated with a severe combined immunodeficiency (SCID) characterized by impairment of both humoral and cell-mediated immunity.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views.

Recommended Usage: Human FFPE: 1:100 dilution. For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References

• Guy, C. S. and Vignali, D. A. Organization of proximal signal initiation at the TCR: CD3 complex. Immunological
  Reviews. 2009; 232: 7-21.

* Conjugate tested on human and mouse FFPE tissue.