Background: CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), plays roles in leukocyte transmigration, angiogenesis, and integrin activation. Specifically, CD31 is highly expressed at endothelial cell-cell junctions, functioning as an adhesion protein to maintain endothelial cell junctional integrity and restore the vascular permeability barrier following inflammatory or thrombotic challenge. The expression of CD31 on endothelial cells makes it a useful marker of the vasculature within the tumor. CD31 can also be found on platelets, monocytes, and granulocytes.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views. These results are pathologist verified.

Recommended Usage: Human FFPE: 1:100 dilution. For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References

• Lertkiatmongkol P., Liao D., Mei H., Hu Y., Newman P.J. Endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31). Curr Opin Hematol. 2016; 23(3):253-9.

* Conjugate tested on human and mouse FFPE tissue.