CD163 Antibody – 142Nd
Catalog: 714204
Clone: D6U1J
Isotype: Rabbit IgG
Reactivity: Human*, Monkey
Application: MIBI-FFPE
Storage: Supplied in antibody stabilizer with 0.05% sodium azide. Store at 4°C
IHC: CD163 antibody staining of FFPE human tonsil
MIBI: CD163 antibody staining (cyan) of FFPE human tonsil, counterstained with dsDNA (magenta)
IHC: CD163 antibody staining of FFPE human liver
MIBI: CD163 staining (cyan) of FFPE human liver, counterstained with dsDNA (magenta)
Background: CD163 is a transmembrane scavenger receptor expressed on the surface of monocytes and macrophages. CD163 is used as a surface marker of M2 type monocytes/macrophages, including M2 type tumor associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression and metastasis.
Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views.
Recommended Usage: Human FFPE: 4 ug/mL dilution. For optimal results, the antibody should be titrated for each desired application.
References
Yoshihiro Komohara et al. Clinical significance of macrophage heterogeneity in human malignant tumors. Cancer Sci. 2014 Jan;105(1):1-8.
* Conjugate tested on human tissue.