Background: The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. β-tubulin, together with α-tubulin, forms heterodimers that serve as the building blocks for microtubules. The structure of microtubules is dynamically regulated in processes related to cell movement, cytoplasmic transport, and chromosome alignment during meiosis/mitosis. β-tubulin is broadly expressed in eukaryotic cells. β-tubulin is the target of several anti-tubulin agents used in the treatment of cancer.

Validation: Each lot of conjugated antibody is quality control tested by staining tissue following the MIBI Staining Protocol optimized for the applicable tissue format with subsequent MIBIscope analysis using the appropriate positive and negative tissue field of views. These results are pathologist verified.

Recommended Usage: Human and Mouse FFPE: 1:100 dilution.
For optimal results, the antibody should be titrated for each desired application.

MIBI technology: Learn more about MIBI™ Technology, a multiplex IHC technology with unmatched sensitivity and true subcellular resolution.

References
Silvia Mihaela Illie et al. Potential clinically useful prognostic biomarkers in triple-negative breast cancer: preliminary results of a retrospective analysis. Breast Cancer (Dove Med Press). 2018; 10: 177–194.

Janke, C. The tubulin code: Molecular components, readout mechanisms, and functions. J Cell Biol. 2014; 206 (4) 461-472.

* Conjugate tested on human and mouse tissue.